Pied de cuve generation for sparkling wines

At LEV2050 we have developed a range of bioreactors to multiply yeasts and other types of microorganisms.

In this video, David García Yoldi, director of LEV2050, explains the functionality of the reactors for sparkling wine. He focuses especially on the process of generating “Pied de cuve” adapted to ethanol for sparkling wines.


The fully automated process of forming vat feet adapted to ethanol led us to receive a patent at international level.

It is a simple process as it is fully automated. It consists of the generation of ethanol-adapted cells in a very progressive way so that we maximise their viability. Wineries can achieve cell counts of between 200 – 350 million cells, totally adapted to ethanol, in 3 days of process in which the reactor works autonomously under the conditions that we indicate.

Thanks to the BR-CV, we can have a foot of vat totally concentrated in cells and highly expressive and aromatic. Therefore, we will have to add very little quantity to the base wine to generate sparkling wines. In this way, the wines are once again limpid (the cells have not suffered metabolic stress) and aromatic, expressive, fruity…

On the first day, the BR-CV bioreactor multiplies the yeasts in a healthy way, we do not want to damage them. Therefore, in this process, as it involves an adaptive process to ethanol, we are going to use another culture medium prepared for this process due to its protection against ethanol: MULTILEV TIRAJE.


Under “Sparkling Process” in the bioreactor, we indicate the hours of multiplication and adaptation in the reactor. Depending on the volume, the bioreactor adapts the conditions of oxygenation and inlet flows of the volume. The second step is to fill with the ingredients specified by the bioreactor.

After that, the process could begin.

On the first day the bioreactor multiplies yeasts with the MULTILEV TIRAGE culture medium, and after the 16-24 hours during which the yeasts multiply, it lowers the temperature (so that toxins do not penetrate) and the oxygen flows.

After that, a peristaltic pump in the reactor would make an automatic and progressive adaptation to ethanol during 48 hours.

In summary, reactors are a necessary, cost-effective and easy-to-use tool, providing high quality, effectiveness, traceability and assurance that the whole process is controlled and that we know exactly how many cells we are adding, as determined by us.


If you would like to know more about our products, our services or about LEV2050, do not hesitate to visit our website, where you can find all the information you are looking for. There you will find all the information you are looking for, we hope you liked it!