{"id":4395,"date":"2020-06-05T08:30:12","date_gmt":"2020-06-05T06:30:12","guid":{"rendered":"https:\/\/www.lev2050.com\/custom-biomass-production\/"},"modified":"2025-12-19T10:16:44","modified_gmt":"2025-12-19T09:16:44","slug":"custom-biomass-production","status":"publish","type":"post","link":"https:\/\/www.lev2050.com\/en\/custom-biomass-production\/","title":{"rendered":"Custom Biomass Production"},"content":{"rendered":"\n

Customized biomass production.<\/strong> <\/h1>\n\n

For custom biomass production<\/a>, the process always starts from a small biomass volume\u2014meaning a defined quantity of microorganisms (either yeasts or lactic acid bacteria) that have been selected and preserved by ultra-freezing at \u221280\u00b0C in 1 mL cryovials. They are then grown on Petri dishes (Image<\/em> 1), and a series of successive scale-ups begins, increasing volume in a controlled manner while maintaining cell concentration and cell viability. <\/p>\n\n

<\/div>\n\n
\"\"
Image 1<\/em>: Yeast culture on a Petri dish.<\/figcaption><\/figure><\/div>\n\n

Maintaining strict hygiene and aseptic conditions is critical to obtain pure culture inocula. Particular attention must also be paid to temperature, agitation, and oxygenation to ensure optimal work under aerobic conditions in a homogeneous medium. Control of all these parameters\u2014together with the use of specific culture media\u2014is essential to producing high-quality inocula. <\/p>\n\n

Once the scale-up stages are completed, biomass is obtained in a cream format, ensuring optimal microorganism vitality and viability.<\/strong> This translates into improved wine organoleptic quality and enhanced fermentation kinetics. <\/p>\n\n

LEV2050 operates a production facility (Image<\/em> 2) with the capacity and versatility<\/strong> to manufacture<\/strong> yeasts, lactic acid bacteria, lysates, and customized nutrients.<\/strong> We carry out strict traceability analyses and controls for viability and contamination to provide our customers with the highest product quality.<\/p>\n\n

<\/div>\n\n
\"\"
Image 2: LEV2050 biomass production facility.<\/em><\/figcaption><\/figure><\/div>\n\n
<\/div>\n\n
Sources<\/h5>\n\n

P\u00e9rez-Torrado, R., G\u00f3mez-Pastor, R., Larsson, C. et al<\/em>. (2009). Fermentative capacity of dry active wine yeast requires a specific oxidative stress response during industrial biomass growth. Applied Microbiology and Biotechnology<\/em>, 81,<\/strong> 951\u2013960. https:\/\/doi.org\/10.1007\/s00253-008-1722-9 <\/a><\/p>\n\n

Vieira, \u00c9rika Dur\u00e3o, Andrietta, Maria da Gra\u00e7a Stupiello, & Andrietta, Silvio Roberto. (2013). Yeast biomass production: a new approach in glucose-limited feeding strategy. Brazilian Journal of Microbiology<\/em>, 44<\/em>(2), 551-558. https:\/\/dx.doi.org\/10.1590\/S1517-83822013000200035<\/a><\/p>\n\n

<\/div>\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

For custom biomass production, the process always begins with a small volume of biomass. This consists of a specific quantity of microorganisms, which can be selected yeasts or lactic acid bacteria, preserved by ultra-freezing at -80\u00b0C in 1 mL cryobials. These are then grown in Petri dishes and [\u2026] <\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"content-type":"","footnotes":""},"categories":[120],"tags":[338,381,416,395,414,402,387,415],"class_list":["post-4395","post","type-post","status-publish","format-standard","hentry","category-englishuncategorized","tag-biomasa-2","tag-biorreactor-2","tag-custom-production","tag-fermentacion-2","tag-inoculum","tag-lactic-acid-bacteria","tag-levadura-2","tag-yeast-multiplication-en"],"_links":{"self":[{"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/posts\/4395","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/comments?post=4395"}],"version-history":[{"count":1,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/posts\/4395\/revisions"}],"predecessor-version":[{"id":4396,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/posts\/4395\/revisions\/4396"}],"wp:attachment":[{"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/media?parent=4395"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/categories?post=4395"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/tags?post=4395"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}