{"id":4367,"date":"2025-11-11T12:19:25","date_gmt":"2025-11-11T11:19:25","guid":{"rendered":"https:\/\/www.lev2050.com\/malolactic-fermentation-in-wine\/"},"modified":"2025-11-11T12:19:25","modified_gmt":"2025-11-11T11:19:25","slug":"malolactic-fermentation-in-wine","status":"publish","type":"post","link":"https:\/\/www.lev2050.com\/en\/malolactic-fermentation-in-wine\/","title":{"rendered":"Malolactic Fermentation in Wine"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">Winemaking demands tight microbiological control, sound process decisions, and rigorous analytical monitoring. Within this framework, malolactic fermentation (MLF) is essential to achieve wines that are stable, balanced, and sensorially consistent. Below, we explain what MLF is, how it is carried out using a sequential post-alcoholic inoculation approach, which factors determine its duration, and what LEV2050 solutions help you execute it with precision.  <\/span><\/p>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-weight: 400;\">What is malolactic fermentation?<\/span><\/h2>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">MLF (malolactic fermentation) is the conversion of L-malic acid into L-lactic acid and CO\u2082, mediated by wine bacteria\u2014primarily Oenococcus oeni as the target species. This transformation reduces the sharp, \u201cgreen\u201d acidity associated with malic acid, improves mouthfeel softness, and enhances microbiological stability in bottle. <br \/>MLF does not generate alcohol: it acts on acid balance and on specific aroma-related compounds (e.g., diacetyl and citrate-derived compounds), which must be managed according to the desired wine style.  <\/span><\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"font-weight: 400;\">Importance in modern winemaking<\/span><\/h3>\n<p><span style=\"font-weight: 400;\">In<\/span> modern <span style=\"font-weight: 400;\"><a href=\"https:\/\/www.lev2050.com\/sector-vitivinicola\/\" target=\"_blank\" rel=\"noopener\">winemaking<\/a><\/span><span style=\"font-weight: 400;\">In modern winemaking, controlled fermentation is a fundamental process. It improves mouthfeel smoothness, balances the gustatory profile, and supports long-term stability. Sensory-wise, it can produce lactic\/buttery notes that add complexity. It also enables acidity modulation based on the desired style, providing greater control during production.   <\/span><\/p>\n<p><span style=\"font-weight: 400;\">MLF also has a key technological role: when completed correctly, it helps prevent refermentation or microbial deviations that could compromise final wine quality. That\u2019s why more and more wineries choose to monitor and steer MLF precisely using selected strains and defined protocols <\/span><\/p>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-weight: 400;\">How is MLF performed using sequential post-alcoholic inoculation?<\/span><\/h2>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">Sequential<\/span> <b>post-alcoholic inoculation<\/b> <span style=\"font-weight: 400;\">means adding<\/span> <i>O. oeni<\/i> <span style=\"font-weight: 400;\">after alcoholic fermentation (AF)<\/span>,<span style=\"font-weight: 400;\"> once low residual sugars and stable process conditions have been confirmed. This approach supports scheduling, SOP standardization, and sensory profile control. <\/span><\/p>\n<p><span style=\"font-weight: 400;\">Typical operational steps:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Confirm AF completion:<\/b> <span style=\"font-weight: 400;\">sugars &lt; 2 g\/L (GLU + FRU) and stable density for 48 h.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Condition the wine:<\/b> <span style=\"font-weight: 400;\">target T = 16\u201320 \u00b0C; free SO\u2082 &lt; 15 mg\/L (adjusted to pH)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Prepare the inoculum:<\/b> <span style=\"font-weight: 400;\">culture\/acclimation of O. oeni under oenological conditions. <\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Inoculate:<\/b> <span style=\"font-weight: 400;\">10\u2076\u201310\u2077 CFU\/mL depending on the matrix, with recirculation and minimal aeration. <\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Monitor:<\/b> <span style=\"font-weight: 400;\">L-malic\/L-lactic acid, pH, and volatile acidity every 48 h.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Close out:<\/b> <span style=\"font-weight: 400;\">once L-malic acid &lt; 0.15 g\/L and the sensory profile meets the target, adjust protective SO\u2082.<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3><i><span style=\"font-weight: 400;\">Oenococcus oeni:<\/span><\/i> <span style=\"font-weight: 400;\">the key player<\/span><\/h3>\n<p><span style=\"font-weight: 400;\">This lactic acid bacterium is responsible for malolactic fermentation. Its action improves stability and the aromatic profile while softening the wine\u2019s palate\u2014making it an essential ally in modern oenology. <\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Wine adaptation:<\/b> <span style=\"font-weight: 400;\">tolerates moderate-to-high ethanol, low pH, and limited nutrient availability.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Wine adaptation:<\/b> <span style=\"font-weight: 400;\">tolerates moderate-to-high ethanol, low pH, and limited nutrient availability.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Requirements:<\/b> <span style=\"font-weight: 400;\">peptides and amino acids, B-group vitamins, and cofactors; yeast autolysis on fine lees can contribute nutrients and survival factors.<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-4353 size-full aligncenter\" title=\"Malolactic fermentation\" src=\"https:\/\/www.lev2050.com\/wp-content\/uploads\/2025\/11\/Fermentacion-malolactica.jpg\" alt=\"Malolactic fermentation\" width=\"1024\" height=\"683\" srcset=\"https:\/\/www.lev2050.com\/wp-content\/uploads\/2025\/11\/Fermentacion-malolactica.jpg 1024w, https:\/\/www.lev2050.com\/wp-content\/uploads\/2025\/11\/Fermentacion-malolactica-300x200.jpg 300w, https:\/\/www.lev2050.com\/wp-content\/uploads\/2025\/11\/Fermentacion-malolactica-768x512.jpg 768w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-weight: 400;\">Lactic acid bacteria in wine<\/span><\/h2>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">In this process, the real protagonists are lactic acid bacteria (LAB).  <\/span>Key species include <i><span style=\"font-weight: 400;\">Oenococcus oeni<\/span><\/i><span style=\"font-weight: 400;\">,<\/span> <i><span style=\"font-weight: 400;\">Lactobacillus<\/span><\/i>, <span style=\"font-weight: 400;\">and<\/span> <i><span style=\"font-weight: 400;\">Pediococcus.<\/span><\/i><span style=\"font-weight: 400;\">. Each brings different traits, but in practice, <\/span><b><i>O. oeni<\/i><\/b> <b>is the most widely used in winemaking due to its ability to withstand typical wine conditions<\/b> <span style=\"font-weight: 400;\">(higher alcohol, low nutrient availability, and low pH).<\/span><\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"font-weight: 400;\">Role in the fermentation<\/span><\/h3>\n<p><span style=\"font-weight: 400;\">LAB transform malic acid into lactic acid, leading to direct consequences: <\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The sharper, more aggressive malic acid is reduced.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The softer, rounder lactic acid improves balance.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Secondary compounds are produced that enrich aroma and flavor.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">This role<\/span> <b>not only improves the sensory profile, but also helps ensure bottle stability.<\/b><span style=\"font-weight: 400;\">. Without LAB activity, undesired fermentations could occur months after bottling, altering the product..<\/span><\/p>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-weight: 400;\">Duration of malolactic fermentation<\/span><\/h2>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">One of the most relevant questions for wineries is how long MLF takes.<\/span> <b>There is no single fixed timeline: it can vary widely depending on wine conditions and technical choices by the winemaker<span style=\"font-weight: 400;\">.<\/span><\/b><\/p>\n<p><span style=\"font-weight: 400;\">In general, the process may last from a few weeks to several months.<\/span><\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"font-weight: 400;\">Factors that influence duration and success <\/span><\/h3>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Temperature:<\/b> <span style=\"font-weight: 400;\">operational optimum<\/span> <b>16\u201320 \u00b0C<\/b> <span style=\"font-weight: 400;\">(adjust to wine style and strain).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Free SO\u2082:<\/b> <span style=\"font-weight: 400;\">keep<\/span> &lt;<b> 10 mg\/L<\/b> <span style=\"font-weight: 400;\">at inoculation; consider molecular SO\u2082 fraction as a function of pH.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>pH:<\/b> <span style=\"font-weight: 400;\">the lower it is (\u2248 3.1\u20133.3), the higher the stress; may require higher inoculation density and acclimation. <\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Alcohol:<\/b> <span style=\"font-weight: 400;\">higher ethanol increases lag time;<\/span> prior <span style=\"font-weight: 400;\">culture acclimation is decisive.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Nutrients:<\/b> <span style=\"font-weight: 400;\">peptide and vitamin availability; well-managed fine lees can help.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Polyphenols:<\/b> <span style=\"font-weight: 400;\">tannic matrices can exert a bacteriostatic effect; consider b\u00e2tonnage or a concentrated pied de cuve (starter). <\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Dissolved CO\u2082:<\/b> <span style=\"font-weight: 400;\">gentle degassing improves enzymatic activity.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Residual sugars:<\/b> <span style=\"font-weight: 400;\">keeping them low reduces risks of increased volatile acidity from hexose consumption and\/or potential refermentation. <\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3><span style=\"font-weight: 400;\">Analytical monitoring and control<\/span><\/h3>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>L-malic \/ L-lactic acid<\/b> <span style=\"font-weight: 400;\">(HPLC or enzymatic kits) every 24\u201348 h.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>pH and volatile acidity:<\/b> <span style=\"font-weight: 400;\">confirm stability and absence of deviations.<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" class=\"aligncenter wp-image-4349 size-full lazyload\" title=\"Lactic acid bacteria\" data-src=\"https:\/\/www.lev2050.com\/wp-content\/uploads\/2025\/11\/Bacterias-lacticas.jpg\" alt=\"Lactic acid bacteria\" width=\"1024\" height=\"683\" data-srcset=\"https:\/\/www.lev2050.com\/wp-content\/uploads\/2025\/11\/Bacterias-lacticas.jpg 1024w, https:\/\/www.lev2050.com\/wp-content\/uploads\/2025\/11\/Bacterias-lacticas-300x200.jpg 300w, https:\/\/www.lev2050.com\/wp-content\/uploads\/2025\/11\/Bacterias-lacticas-768x512.jpg 768w\" data-sizes=\"(max-width: 1024px) 100vw, 1024px\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" style=\"--smush-placeholder-width: 1024px; --smush-placeholder-aspect-ratio: 1024\/683;\" \/><\/p>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-weight: 400;\">Differences between lactic fermentation and malolactic fermentation<\/span><\/h2>\n<p>&nbsp;<\/p>\n<h3><span style=\"font-weight: 400;\">Lactic fermentation in wine<\/span><\/h3>\n<p><span style=\"font-weight: 400;\">This is a biological process in which LAB (such as Lactobacillus or Leuconostoc) convert sugars (glucose, fructose) into lactic acid, increasing acidity and potentially producing sour or vinegar-like notes.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Therefore, in wine it is generally undesirable; it can occur in spoiled\/altered wines due to bacterial contamination or poor winery hygiene.<\/span><\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"font-weight: 400;\"> Malolactic fermentation (MLF)<\/span><\/h3>\n<p><span style=\"font-weight: 400;\">As noted, this is a secondary, controlled fermentation also carried out by LAB (mainly Oenococcus oeni), but it does not convert sugars. Instead, it converts malic acid into lactic acid and CO\u2082.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">This process softens wine acidity and improves microbiological stability. The outcome: <\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Acidity decreases (malic acid has two protons; lactic acid has one).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">A rounder, creamier mouthfeel is achieved.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Secondary aromas are generated (butter, cream, nuts).<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-weight: 400;\">LEV2050 solutions to execute sequential MLF with precision<\/span><\/h2>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">At LEV2050, we develop specific tools so that post-alcoholic MLF becomes predictable, efficient, and traceable within an industrial microbiology framework.<\/span><\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"font-weight: 400;\">LACTILEV OENI \u2014 Culture medium for Oenococcus oeni<\/span><\/h3>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Objective:<\/b> <span style=\"font-weight: 400;\">growth, multiplication, and acclimation of O. oeni under oenological conditions (pH and ethanol).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Operational results:<\/b> <span style=\"font-weight: 400;\">highly viable, robust inocula for wine addition, reducing lag phase and improving process continuity<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3><span style=\"font-weight: 400;\">BR-LEV-LC\/CV\u00ae \u2014 Bioreactors for O. oeni cultivation and scale-up<\/span><\/h3>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Practical advantages:<\/b> <span style=\"font-weight: 400;\">consistent pied de cuve preparation, fast starts, and rapid implantation in wine<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><b>Economic advantages:<\/b> <span style=\"font-weight: 400;\">70\u201380% lower use of lactic bacteria.<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">In short, sequential post-alcoholic malolactic fermentation driven by Oenococcus oeni requires well-acclimated inocula, stable process parameters, and rigorous analytical monitoring. With LACTILEV OENI and BR-LEV-LC\/CV\u00ae bioreactors, LEV2050 enables a robust, traceable, and highly cost-efficient technical implementation aligned with each winery\u2019s sensory and stability goals.<\/span><\/p>\n<p><a href=\"https:\/\/www.lev2050.com\/contacto\/\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\">Shall we talk?<\/span><\/a><span style=\"font-weight: 400;\"> Our technical team can size the culture, define the inoculation SOP, and design the analytical plan for your wine and working conditions.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Winemaking demands tight microbiological control, sound process decisions, and rigorous analytical monitoring. Within this framework, malolactic fermentation (MLF) is essential to achieve wines that are stable, balanced, and sensorially consistent. Below, we explain what MLF is, how it is carried out using a sequential post-alcoholic inoculation approach, which factors determine its duration, and what LEV2050 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":"","footnotes":""},"categories":[391],"tags":[],"class_list":["post-4367","post","type-post","status-publish","format-standard","hentry","category-espanol-2"],"_links":{"self":[{"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/posts\/4367","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/comments?post=4367"}],"version-history":[{"count":0,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/posts\/4367\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/media?parent=4367"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/categories?post=4367"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lev2050.com\/en\/wp-json\/wp\/v2\/tags?post=4367"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}